| Seed Line | Seed | Team |
| 1 | 1 | Auburn |
| 1 | 2 | Houston |
| 1 | 3 | Duke |
| 1 | 4 | Florida |
| 2 | 5 | Tennessee |
| 2 | 6 | Alabama |
| 2 | 7 | Michigan St. |
| 2 | 8 | St. John’s ⬆️ |
| 3 | 9 | Texas Tech ⬇️ |
| 3 | 10 | Kentucky |
| 3 | 11 | Iowa St. |
| 3 | 12 | Wisconsin ⬆️ |
| 4 | 13 | Texas A&M ⬇️ |
| 4 | 14 | Maryland |
| 4 | 15 | Michigan |
| 4 | 16 | Arizona ⬆️ |
| 5 | 17 | Oregon |
| 5 | 18 | Purdue ⬇️ |
| 5 | 19 | Louisville |
| 5 | 20 | Clemson |
| 6 | 21 | Illinois |
| 6 | 22 | Ole Miss |
| 6 | 23 | BYU ⬆️ |
| 6 | 24 | Saint Mary’s |
| 7 | 25 | UCLA ⬇️ |
| 7 | 26 | Kansas |
| 7 | 27 | Missouri |
| 7 | 28 | Gonzaga |
| 8 | 29 | Marquette |
| 8 | 30 | Memphis |
| 8 | 31 | Mississippi St. |
| 8 | 32 | Creighton |
| 9 | 33 | Georgia |
| 9 | 34 | Connecticut |
| 9 | 35 | Oklahoma ⬆️⬆️ |
| 9 | 36 | New Mexico |
| 10 | 37 | Baylor |
| 10 | 38 | Utah St. |
| 10 | 39 | Arkansas |
| 10 | 40 | Drake ⬆️ |
| 11 | 41 | West Virginia ⬇️⬇️ |
| 11 | 42 | Vanderbilt ⬇️ |
| 11 | 43 | Indiana |
| 11 | 44 | San Diego State |
| 11 | 45 | VCU |
| 11 | 46 | Texas 🆕 |
| 12 | 47 | UC San Diego |
| 12 | 48 | Colorado State 🆕 |
| 12 | 49 | Liberty |
| 12 | 50 | McNeese St. |
| 13 | 51 | Yale |
| 13 | 52 | High Point |
| 13 | 53 | Akron |
| 13 | 54 | Grand Canyon 🆕 |
| 14 | 55 | Lipscomb ⬇️ |
| 14 | 56 | Troy |
| 14 | 57 | UNC Wilmington |
| 14 | 58 | Montana 🆕 |
| 15 | 59 | Robert Morris |
| 15 | 60 | Wofford |
| 15 | 61 | Norfolk State |
| 15 | 62 | Bryant ⬆️ |
| 16 | 63 | Omaha |
| 16 | 64 | Mount St. Mary’s 🆕 |
| 16 | 65 | SIU Edwardsville |
| 16 | 66 | American |
| 16 | 67 | Alabama St. |
| 16 | 68 | Saint Francis (PA) 🆕 |
| Last 4 Byes | Last 4 In | First 4 Out | Next 4 Out |
| Baylor ⬇️ | Vanderbilt ⬇️ | North Carolina ⬇️ | Nebraska ⬇️ |
| Utah State | Indiana | Xavier | Wake Forest |
| Arkansas | San Diego St. | Ohio St. ⬇️ | San Francisco ⬆️ |
| West Virginia ⬇️ | Texas ⬆️ | Boise St. | UC Irvine ⬆️ |
Shoutout to www.bracketmatrix.com for keeping track of all of the brackets over the years!